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| 6. |  | DOS SANTOS-NETO, P.C.; CUADRO, F.; SOUZA-NEVES, M.; CRISPO, M.; MENCHACA, A. Refinements in embryo manipulation applied to CRISPR technology in livestock. Theriogenology. 2023, Volume 208, Pages 142-148. https://doi.org/10.1016/j.theriogenology.2023.05.028 Article history: Received 14 April 2023; Received in revised form 29 May 2023; Accepted 29 May 2023; Available online 9 June 2023. -- Correspondence author: Menchaca, A.; Instituto de Reproducción Animal Uruguay, Fundación IRAUy,...| Biblioteca(s): INIA Las Brujas. |
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| 7. | | CUADRO, F.; DE BRUN, V.; BROCHADO, C.; SOUZA, M.; GARCÍA PINTOS, C.; MENEZES, C.; GASTAL, G.D.A.; MENCHACA, A. Gene expression patterns in uterus and oviduct during the preovulatory period in ewes. In: 19th International Congress on Animal Reproduction (ICAR), Bologna (Italy), 26-30 June 2022. p.185.| Biblioteca(s): INIA La Estanzuela. |
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| 8. | | CRISPO, M.; CHENOUARD, V.; DOS SANTOS-NETO P; TESSON, L.; SOUZA-NEVES, M.; HESLAN, J.M.; CUADRO, F.; ANEGÓN, I.; MENCHACA, A. Generation of a Human Deafness Sheep Model Using the CRISPR/Cas System. Chapter 12. Methods in Molecular Biology, 2022, Volume 2495, Pages 233-258. doi: https://doi.org/10.1007/978-1-0716-2301-5_12| Biblioteca(s): INIA La Estanzuela. |
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| 9. | | COSTALES, A.; DARING, E.; MELO, S.; ROMERO, IBER; GIORELLO, NÉSTOR; PRADIÉ, W.; CARDOZO, G.D.; OLIVERA, A.L.; LEAL, K.; DUTRA, V.; CUADRO, F.; LEMA, J.; LEONI, C.; URANGA, M.Z. Incidencia del raleo manual en la calidad final de la fruta en duraznero. Tacuarembó: s.e., 1997. 67 p. Trabajo realizado por alumnos de 1er. año liceal en convenio con INIA Tacuarembó.| Biblioteca(s): INIA Tacuarembó. |
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| 10. | | PASSOS, J. R. S.; GUERREIRO, D. D.; OTÁVIO, K. S.; SANTOS-NETO, P. C. DOS; SOUZA-NEVES, M.; CUADRO, F.; NUÑEZ-OLIVERA, R.; CRISPO, M.; BEZERRA, M. J. B.; SILVA, R. F.; LIMA, L. F.; FIGUEIREDO, J. R.; BUSTAMANTE-FILHO, I. C.; MENCHACA, A.; MOURA, A. A. Global proteomic analysis of preimplantational ovine embryos produced in vitro. Reproduction in Domestic Animals, 2022, Volume 57, Issue 7; pages 784-797. doi: https://doi.org/10.1111/rda.14122 Article history: Received 15 February 2022; Accepted 1 April 2022. -- Funding text - The experiments presently described were conducted at the facilities of the (Fundacion IRAUy, Montevideo, Uruguay) and at the (UBAL) of the , Uruguay....| Biblioteca(s): INIA Las Brujas. |
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| 11. | | PASSOS, J.R.S.; GUERREIRO, D.D.; KAMILA S. OTÁVIO; DOS SANTOS-NETO, P.C.; SOUZA-NEVES, M.; CUADRO, F.; NUÑEZ?OLIVERA, R.; CRISPO, M.; VASCONCELOS, F.R.; BEZERRA, M.J.B.; SILVA, R.F.; LIMA, L.F.; FIGUEIREDO, J.R.; BUSTAMANTE-FILHO, I.C.; MENCHACA, A.; MOURA, A.A. How in vitro maturation changes the proteome of ovine cumulus-oocyte complexes?. Volume 89, Issue 10, Pages 459 - 470October 2022 Molecular reproduction and development, October 2022, Volume 89, Issue 10, pages 459-470. doi: https://doi.org/10.1002/mrd.23638 Article history: Received: 16 February 2022 | Accepted: 21 July 2022. -- Corresponding author: Moura, A.A.; Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil;...| Biblioteca(s): INIA La Estanzuela. |
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| Registros recuperados : 11 | |
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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
20/07/2022 |
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Actualizado : |
20/07/2022 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
CRISPO, M.; CHENOUARD, V.; DOS SANTOS-NETO P; TESSON, L.; SOUZA-NEVES, M.; HESLAN, J.M.; CUADRO, F.; ANEGÓN, I.; MENCHACA, A. |
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Afiliación : |
MARTINA CRISPO, Laboratory Animal Biotechnology Unit, Institut Pasteur de Montevideo, Montevideo, Uruguay.; VANESSA CHENOUARD, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; PEDRO DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; LAURENT TESSON, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; MARCELA SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; JEAN-MARIE HESLAN, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; FEDERICO CUADRO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; IGNACIO ANEGÓN, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay. |
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Título : |
Generation of a Human Deafness Sheep Model Using the CRISPR/Cas System. |
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Complemento del título : |
Chapter 12. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Methods in Molecular Biology, 2022, Volume 2495, Pages 233-258. doi: https://doi.org/10.1007/978-1-0716-2301-5_12 |
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DOI : |
10.1007/978-1-0716-2301-5_12 |
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Idioma : |
Inglés |
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Contenido : |
Abstract:
CRISPR/Cas9 system is a promising method for the generation of human disease models by genome editing in non-conventional experimental animals. Medium/large-sized animals like sheep have several advantages to study human diseases and medicine. Here, we present a protocol that describes the generation of an otoferlin edited sheep model via CRISPR-assisted single-stranded oligodinucleotide-mediated Homology-Directed Repair (HDR), through direct cytoplasmic microinjection in in vitro produced zygotes.Otoferlin is a protein expressed in the cochlear inner hair cells, with different mutations at the OTOF gene being the major cause of nonsyndromic recessive auditory neuropathy spectrum disorder in humans. By using this protocol, we reported for the first time an OTOF KI model in sheep with 17.8% edited lambs showing indel mutations, and 61.5% of them bearing knock-in mutations by HDR . The reported method establishes the bases to produce a deafness model to test novel therapies in human disorders related to OTOF mutations. |
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Palabras claves : |
Embryo; Genome editing; In vitro fertilization; OTOF; SsODNs. |
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Thesagro : |
OVEJA. |
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Asunto categoría : |
-- |
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Marc : |
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245 $aGeneration of a Human Deafness Sheep Model Using the CRISPR/Cas System.$h[electronic resource]
260 $c2022
520 $aAbstract: CRISPR/Cas9 system is a promising method for the generation of human disease models by genome editing in non-conventional experimental animals. Medium/large-sized animals like sheep have several advantages to study human diseases and medicine. Here, we present a protocol that describes the generation of an otoferlin edited sheep model via CRISPR-assisted single-stranded oligodinucleotide-mediated Homology-Directed Repair (HDR), through direct cytoplasmic microinjection in in vitro produced zygotes.Otoferlin is a protein expressed in the cochlear inner hair cells, with different mutations at the OTOF gene being the major cause of nonsyndromic recessive auditory neuropathy spectrum disorder in humans. By using this protocol, we reported for the first time an OTOF KI model in sheep with 17.8% edited lambs showing indel mutations, and 61.5% of them bearing knock-in mutations by HDR . The reported method establishes the bases to produce a deafness model to test novel therapies in human disorders related to OTOF mutations.
650 $aOVEJA
653 $aEmbryo
653 $aGenome editing
653 $aIn vitro fertilization
653 $aOTOF
653 $aSsODNs
700 1 $aCHENOUARD, V.
700 1 $aDOS SANTOS-NETO P
700 1 $aTESSON, L.
700 1 $aSOUZA-NEVES, M.
700 1 $aHESLAN, J.M.
700 1 $aCUADRO, F.
700 1 $aANEGÓN, I.
700 1 $aMENCHACA, A.
773 $tMethods in Molecular Biology, 2022, Volume 2495, Pages 233-258. doi: https://doi.org/10.1007/978-1-0716-2301-5_12
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